Director: Allen W. Cowley, Jr, Ph.D.
Supervisors: Carol Moreno Quinn, M.D., Ph.D.
Co-Investigators: Thom Feroah, Ph.D., Andrew Greene, Ph.D, Carol Moreno Quinn, M.D., Ph.D, Hubert Forster,
Ph.D., Julian Lombard, Ph.D., David Mattson, Ph.D., Abraham Provoost,
Ph.D., and Richard Roman, Ph.D.
Component Contact person: Carol Moreno Quinn, M.D., Ph.D.
The Phenotyping Component
provides rapid, high throughput, precise and detailed phenotypic
measurements in consomic rat strains, ENU mutant rat strains and their respective parental
The data will provide the functional information
required to link each of the 22 rat chromosomes to cardiovascular,
pulmonary, renal, and blood function using the strategy of chromosomal
substitution. The ENU mutant strategy allows us to knock-out genes
in disease backgrounds as well as “normal” models, thus narrowing the
search for genes in pathways that participate in disease processes
involving the heart, lung, blood, vasculature and kidneys.
Experiments will be performed on 5 male and 5 female rats of each ENU mutant rats strain. Two male FHH rats will be run with each ENU mutant strain for quality control. Each parental strain (SS, BN & FHH) will be screened using the same protocols used for the ENU mutant strains. The screening protocol will obtain physiological measurements of heart, renal, vascular, movement/behavior and blood function. This figure (click it to zoom) provides a summary of the protocol timeline.
Click here for a summary
of the protocol timeline.
Experiments will be
performed on consomic strains from the SS, BN, & FHH parental
strains (and others time permitting). Two male rats of the parental
strain with the “control” genomic background (e.g., for SS.BN consomic,
SS parental strain is the control) will be run with each consomic
strain. Each strain will enter the protocols allowing for the functional
characterization of both genders and parental control rats.
The different protocols will obtain 214 physiological measurements
of heart, lung, vascular, and blood function (airway responsiveness,
blood pressure regulation, lung function, and renal function in
response to acute and chronic hypoxia, exercise, and chronic changes
in salt diet).
strain will enter the protocols allowing for the functional characterization
of both genders and parental control rats over a 5 week period.
This figure (click it to zoom) shows the course of the 5 week protocol.
Click on the Protocols menu on the navigation
bar of the left side to view details.