Announces the Availability of Mutant Rats
PhysGen, one of five Programs
for Genomic Applications (PGA) funded by
the National Heart, Lung and Blood Institute is currently generating
knockout rats using an ENU (N-ethyl-N-nitrosourea) mutagenesis strategy.
Male rats were subjected to ENU and bred to healthy female rats
to generate F1 heterozygous offspring. Prior to phenotypic screening,
the offspring are screened by TILLING, a high-throughput method
which identifies mutations by enzyme cleavage of heteroduplex PCR
product formation between wild-type and mutated alleles. During
the screening process, several amino acid changes were identified
in targeted genes. Confirmed gene knockouts will be phenotypically
characterized and data will be released on the PhysGen website.
We are pleased to make the rats with amino acid changes available
to interested investigators (click here for details). Heterozygous
mutant rats (5M/5F) will be available on a first come-first serve
basis for 75 days after posting on PhysGen's website (http://pga.mcw.edu).
For additional information on how to obtain these rats, please contact
Melinda Dwinell at (414) 456-4498 (email@example.com).
Completion of consomic development and characterization
PhysGen has completed the development and physiological characterization
of two panels of consomic rat strains (SSxBN, FHHxBN). High-throughput phenotypic screens of cardiovascular, pulmonary, renal, vascular and blood systems have been completed for all 44 consomic rat strains as well as 11 commonly used rat strains. Physiological data can be viewed by clicking here
Rat Genomics and Models
Date: Dec. 6-9, 2007
Location: Cold Spring Harbor Laboratory
Additional Information: here
Archived News and Events
GENOMICS & MODELS, December 8-11, 2005, Cold Spring
Harbor Laboratory Organizers:
Timothy Aitman, Imperial College,
Howard Jacob, Medical College of
James Shull, University of Nebraska
BIOLOGY 2006 , April 1-5, 2006, San Francisco, CA
PhysGen & Rat Genome Database will host
a booth (#1317), "Rat Physiological Genomic Tools"
Nov 3, 2004:
The Programs for Genomic Applications (PGA) grant renewal was awarded to the Medical College of Wisconsin, Howard Jacob Ph.D., Principal Investigator, on July 29, 2004. The grant renewal, Knock-Out Rats for Physiological Genomics, will extend from 08/01/2004 to 07/31/2008.
On September 30, 2000, the National Heart, Lung, and Blood Institute (NHLBI) launched the Programs for Genomic Applications (PGAs) http://www.nhlbi.nih.gov/resources/pga/. This program is a major initiative to advance functional genomic research related to heart, lung, blood, and sleep health and disorders. The MCW program, termed PhysGen, has been focused on understanding the genetic basis of fundamental mechanistic pathways of the heart, lung, kidney, blood and vasculature through development of consomic rat panels and physiological genomics, using environmental stressors.
The PhysGen renewal will continue the success of the program by capitalizing on a variety of technological advances, extending the data by manipulating specific genes and assessing the results physiologically in three different genetic backgrounds. The "functionality" of relevant genes will be tested by generating knockouts (KO) in rats, using a novel strategy with ENU mutagenesis. (Large-Scale Discovery of Induced Point Mutations With High-Throughput TILLING. Genome Res., Mar 2003; 13: 524 - 530; Production of knockout rats using ENU mutagenesis and a yeast-based screening assay. Yunhong Zan, Jill D Haag, Kai-Shun Chen, Laurie A Shepel, Don Wigington, Yu-Rong Wang, Rong Hu, Christine C Lopez-Guajardo, Heidi L Brose, Katherine I Porter, Rachel A Leonard, Andrew A Hitt, Stacy L Schommer, Anu F Elegbede & Michael N Gould. Nature Biotechnology 21, pp 645 - 651, 2003).
The genes selected for KO (Click here to see gene list) have been derived from those that have been linked to heart, lung and blood disorders using molecular genetic, genomic and expression technologies during the first four years of all PGA programs. As genomic background is shown to play a major role in the expression of phenotypes in KO mice, this PGA will attempt to make the KOs in 3 genetic backgrounds in the rat, the SS, BN, and FHH strains. The selection of these 3 strains will also enable PhysGen to leverage the enormous amount of phenotypic data generated on these strains during the first four years, notably 8,610 physiological data points per strain, including baseline and stressors, providing an unprecedented level of baseline data for a mutagenesis screen.
If you would like to comment on the list, or suggest additional genes for consideration please contact Julie Messinger at (414) 456-4531 or firstname.lastname@example.org
16, 2004: Parental, consomic and congenic strain
profiles are now available. The strain profile includes general
information for each rat strain, as well as summaries and
statistical analysis of significant phenotypes. Click
here for details. Stay tuned for more.
March 31, 2004
- An international research team, supported by the National Institutes
of Health (NIH), announced it has completed a high-quality, draft
sequence of the genome of the Brown Norway rat http://www.nhgri.nih.gov/11511308.
The strain is the BN/NHsdMcwi, which has been extensively
characterized by PhysGen. Click
here to select and review phenotype, genotype, microarray or
strain profile data on the PhysGen website.
Click here for old news.
Rat genome sequence: BN/NHsd/Mcwi draft sequence is available on
UCSC genome browser