PhysGen General Information


ENU Data

The phenotyping The screening protocol for the ENU mutant rats provides 84 phenotypic measurements of heart, lung, vascular, renal and blood measurements. All measurements are made in each rat over a three week period. Both male and female rats are studied to screen for gender differences. All rats are put through the movement & respiratory protocol while on a low salt diet (Purina 5001). After this part of the screening protocol, the mutant rats with BN or FHH genetic background are switched to a high salt (4% Dyets) diet for two weeks prior to the remainder of the screening protocol. The mutant rats with a SS genetic background are kept on a low salt diet for the entire screening protocol.

  ENU Strain

  ENU Protocol

View the complete rat strain nomenclature.

The description of the Phenotyping Protocols.

  ENU Conditioning

The description of the diet and hypoxia conditioning stimuli.



Consomic Data

The phenotyping component provides 213 phenotypic measurements of heart, lung, vascular, and blood function. Each strain enters the protocols allowing for the functional characterization of both genders and parental control rats over a 5-week period. The data will provide the functional information required to link each of the 21 rats chromosomes to cardiovascular, pulmonary, renal and blood function.

  Strain

  Protocol

View the complete rat strain nomenclature.

The description of the Phenotyping Protocols.

  Conditioning Stimuli

  Timeline

The description of the diet and hypoxia conditioning stimuli.

View the timing projection for strain development and phenotyping. Also listed are the strains that have been phenotyped.

  Quality Assurance for Phenotypic Data

  Quality Assurance for Genomics Component

The description of the QA procedures used in the phenotyping component.
(Click here for PDF document).

The description of the QA procedures used in the genomics component.
(Click here for PDF document).

  Quality Assurance for Phenotypic Data

  Quality Assurance for Genomics Component

The description of the QA procedures used in the phenotyping component.
(Click here for PDF document).

The description of the QA procedures used in the genomics component.
(Click here for PDF document).

  Genotyping Protocol Descriptions

  PhysGen Resource Distribution Policy

View the policy.