Phenotyping Component


Director: Allen W. Cowley, Jr, Ph.D.
Supervisors: Carol Moreno Quinn, M.D., Ph.D.
Co-Investigators: Thom Feroah, Ph.D., Andrew Greene, Ph.D, Carol Moreno Quinn, M.D., Ph.D, Hubert Forster, Ph.D., Julian Lombard, Ph.D., David Mattson, Ph.D., Abraham Provoost, Ph.D., and Richard Roman, Ph.D.
Component Contact person: Carol Moreno Quinn, M.D., Ph.D.

Description

The Phenotyping Component provides rapid, high throughput, precise and detailed phenotypic measurements in consomic rat strains, ENU mutant rat strains and their respective parental strains. The data will provide the functional information required to link each of the 22 rat chromosomes to cardiovascular, pulmonary, renal, and blood function using the strategy of chromosomal substitution. The ENU mutant strategy allows us to knock-out genes in disease backgrounds as well as “normal” models, thus narrowing the search for genes in pathways that participate in disease processes involving the heart, lung, blood, vasculature and kidneys.

Protocols

ENU Phenotyping

Experiments will be performed on 5 male and 5 female rats of each ENU mutant rats strain. Two male FHH rats will be run with each ENU mutant strain for quality control. Each parental strain (SS, BN & FHH) will be screened using the same protocols used for the ENU mutant strains. The screening protocol will obtain physiological measurements of heart, renal, vascular, movement/behavior and blood function. This figure (click it to zoom) provides a summary of the protocol timeline. Click here for a summary of the protocol timeline.

Consomic Phenotyping

Experiments will be performed on consomic strains from the SS, BN, & FHH parental strains (and others time permitting). Two male rats of the parental strain with the “control” genomic background (e.g., for SS.BN consomic, SS parental strain is the control) will be run with each consomic strain. Each strain will enter the protocols allowing for the functional characterization of both genders and parental control rats. The different protocols will obtain 214 physiological measurements of heart, lung, vascular, and blood function (airway responsiveness, blood pressure regulation, lung function, and renal function in response to acute and chronic hypoxia, exercise, and chronic changes in salt diet).

Each strain will enter the protocols allowing for the functional characterization of both genders and parental control rats over a 5 week period. This figure (click it to zoom) shows the course of the 5 week protocol.

Protocol Manuals

Click on the Protocols menu on the navigation bar of the left side to view details.

Components
Genomics
Phenotyping
Research Services
Bioinformatics
Education
Administration