Diet & Atmosphere Condition Description


RENAL PROTOCOLS

Renal Protocol A:
Protocol A was designed to characterize arterial blood pressure, blood pressure-related phenotypes, and the susceptibility to developing renal injury in response to a high salt diet in 12-week old rats. Protocol duration was 2 weeks.
Diet
Rats were weaned onto low salt, 0.4% NaCl, diet (Dyets or Teklad, depending on the date of the study) and switched to high salt, 4.0% NaCl, diet (Dyets or Teklad, depending on the date of the study) 21 days prior to study; Atmosphere - Normoxia. Diet of the parents was low salt, 0.4% NaCl, (either Teklad or Dyets, depending on the date of the study). In addition, FHH.BN consomic and appropriate parental groups received the nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester (L-NAME; 25mg/L) in drinking water for 21 days prior to the phenotyping protocol.

Supplier Catalog order # Percent NaCl
Dyets Inc. 113755 0.4
Dyets Inc. 113756 4.0
Teklad 3075S 0.4
Teklad TD01454 4.0

Renal Protocol B:
Protocol B was then designed to provide a standardized reevaluation of all rat strains that had been previously studied. The protocol was therefore streamlined into a 1-week study. Differences in phenotype occurred that were dependent upon the diet provided to the rats. For example, rats fed 4.0% NaCl Dyets became more hypertensive and proteinuric than rats fed 4.0% Teklad. These differences were discovered when diets were changed in an effort to improve the efficiency of breeding.
Diet
Rats are weaned onto low salt diet (0.4% NaCl; Dyets) and switched to high salt diet (8.0% NaCl; Dyets) 17 days prior to study; atmosphere - Normoxia. Diet of the parents is 0.4% NaCl Teklad chow. In addition, FHH.BN consomic and appropriate parental groups receive the nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester (L-NAME; 12mg/L) in drinking water for 17 days prior to the phenotyping protocol.

Supplier Catalog order # Percent NaCl
Dyets Inc. 113755 0.4
Dyets Inc. 100078 8.0
Teklad 3075S 0.4

Please see http://www.teklad.com/index.htm and http://www.dyets.com/dietcom.htm for more information.