Screening protocol for ENU mutant rats

The screening protocol for the ENU mutant rats provides 84 phenotypic measurements of heart, lung, vascular, renal and blood measurements. All measurements are made in each rat over a three week period. Both male and female rats are studied to screen for gender differences. All rats are put through the movement & respiratory protocol while on a low salt diet (Purina 5001). After this part of the screening protocol, the mutant rats with BN or FHH genetic background are switched to a high salt (4% Dyets) diet for two weeks prior to the remainder of the screening protocol. The mutant rats with a SS genetic background are kept on a low salt diet for the entire screening protocol. The following are the brief descriptions of each part of the screening protocol. For detailed information about each screening protocol, click on the link within the protocol description.

Protocol NameProtocol DescriptionNum. of Phenotypes
Vascular responsiveness to vasoconstrictors and vasodilators are measured through an aortic ring preparation in 11 week old rats on a low salt (SS background) or high salt (BN and FHH background) diet.Click here for details9
A blood sample is taken from 11 week old rats following a 16 hour fast to characterize clinical chemistry and hematology in ENU mutant rats. Click here for details29
Cardiac structure and functional measurements are made in 11 week old rats under control conditions and following dobutamine infusion.Click here for details33
Measurement of body weight, body length, and organ weights are measured in 11 weeks old rats.Click here for details7
Blood pressure and related phenotypes are measured in 11 week old rats on a low salt (SS background) or high salt (BN and FHH background) diet. Urine analysis, blood pressure measurement and water consumption provide insight into the role of the kidneys in blood pressure regulation in the ENU mutant rats.Click here for details11
The MOVEMENT protocol is to characterize the movement and behavior responses of the different mutant and consomic strains of rats to a novel environment (first 6 hours) and the behavior, movement and breathing circadian changes over 2.5 days within the plethysmographic chambers. In addition, the final purpose of the protocol is to characterize the ventilatory response to hypercapneia.Click here for details22